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By Samols D., Agrawal A., Kushner I.

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24 3 Cell-Based Biosensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 Bacterial Cells vs Mammalian Cells . . . . . . . . . . . . . . . . . . . . . . . . . 27 4 Classification of Mammalian CBBs . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 CBBs Utilizing Electrical Measurements . . . . .

The sentinel animal species have been successfully used for environmental monitoring applications, hazard and risk assessments, and detection and screening of deleterious changes in animal and human ecosystems [26]. Even though the use of animals could provide useful information about physiological risks associated with a particular agent or groups of agents for a prolonged period of time, the limited portability and robustness for practical large scale application for in-field or military applications preclude the use of large living animals for biosensing.

7 CBBs Using a 3D Cell Culture System . . . . . . . . . . . . . . . . . . . . . . . 8 Current Status of Cell-Based Biosensing . . . . . . . . . . . . . . . . . . . . . . 42 5 Limitations and Drawbacks of CBBs . . . . . . . . . . . . . . . . . . . . . . . . . . . 45 6 Concluding Remarks and Future of CBBs . . . . . . . . . . . . . . . . . . . . .

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