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Additional resources for Bioactive natural Products (Part D)
Aeruginosa and E. coli. Both SAMM2 and SAM MMl had no antibacterial activities. Table 5 illustrates these results. In conclusion, antifungal/antibacterial activities evaluation revealed that, sampangine metabolites have remarkable in vitro antifungal activities, whereas SAMM2 is inactive in vivo in the mouse model of cryptococcosis. This led us to conclude that metabolism per se is not the cause for the in vivo inactivity of sampangine, hence, further studies on the other pharmacokinetic parameters (bioavailability, distribution, clearance, etc) will be necessary.
This metabolite (designated SAMM2) was more polar than sampangine. By this time the culture turned into a bright red color and no deposited sampangine was seen on the wall of the fermentation flasks. The fermentation process was terminated and the incubation mixtures were combined, filtered to remove the mycelia, and the filtrate was extracted with water-saturated butanol (4Lx4). 54g). 5) mixture as OCHq SAMMl (Sampangine-4'-0-methyl-p-glucopyranoside) OH OH SAMM2 (Sampangine-P-glucopyranoside) COOH OH SAMMMI (Sampangine-p-glucuronic acid) BZSAMMl (Benzosampangine-p-gluycopyranoside) 26 KHALED Y.
Streptomyces spectabilis (ATCC 27465) and Streptomyces gnseus(ATCC 13968) were chosen for screening studies to evaluate their demethylation ability based on a published study done by Hufford et al . In that study 5. griseus and S, spectabilis were shown to be capable of demethylating colchicine into several demethylated metabolites. They were shown to do so, only when grown in medium 6 which consists of (per liter of distilled water): glucose, 10 g; com steep liquor, 10 g; soya flour, 10 g; dry malt extract, 5 g; CaCOs, 1 g; NaCl, 5 g.