By Andy Beaumont, Pierre Boudry, Kathrin Hoare(auth.)
Following the tremendous well-received constitution of the 1st version, this rigorously revised and up to date re-creation now contains a lot new details of significant significance to these operating and studying within the fisheries and aquaculture industries.
beginning with chapters masking genetic edition and the way it may be measured, the authors then examine genetic constitution in typical populations, through a brand new bankruptcy overlaying genetics when it comes to inhabitants measurement and conservation matters. Genetic version of characteristics and triploids and the manipulation of ploidy are totally lined, and one other new bankruptcy is integrated, entitled 'From Genetics to Genomics'. The publication concludes with a bankruptcy masking the effect of genetic engineering in aquaculture.
With the inclusion of a wealth of updated details, new textual content and figures and the inclusion of a 3rd writer, Pierre Boudry, the second one variation of Biotechnology and Genetics in Fisheries and Aquaculture offers an outstanding textual content and reference of serious worth and use to top point scholars and pros operating throughout fish biology, aquatic sciences, fisheries, aquaculture, genetics and biotechnology. Libraries in all universities and study institutions the place organic sciences, fisheries and aquaculture are studied and taught must have numerous copies of this glorious new version on their cabinets.
- Completely up to date, revised and multiplied new version
- Subject quarter of ever expanding significance
- Expanded authorship
- Commercially beneficial info for fish breeders
Chapter 1 what's Genetic edition? (pages 1–18):
Chapter 2 How can Genetic version be Measured? (pages 19–48):
Chapter three Genetic constitution in common Populations (pages 49–76):
Chapter four Genetics of inhabitants dimension in Conservation and Aquaculture (pages 77–97):
Chapter five Genetic version of characteristics (pages 99–128):
Chapter 6 From Genetics to Genomics (pages 129–143):
Chapter 7 Triploids and past: Why manage Ploidy? (pages 145–160):
Chapter eight Genetic Engineering in Aquaculture (pages 161–180):
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Extra info for Biotechnology and Genetics in Fisheries and Aquaculture, Second Edition
We have shown how we can determine the precise sequence of a length of DNA, and how it varies between individuals. Now we shall progress to see how differences between sizes of DNA fragments can be identified and used to address particular genetic questions. Techniques that fall into this category include those known by the acronyms RFLP, VNTR, DNA fingerprinting, RAPD and AFLP. Of these, VNTR markers (microsatellites in particular) have come to the fore in recent years as being the most generally useful, though the others all have their place in answering particular genetic questions.
5). 5 Alignment of sequences is used to identify single nucleotide polymorphisms and Indels. 1 = transition, 2 = transversion, 3 = indel. SNPs occur every 1000 bases or so in the human genome, but can be much more frequent in many marine species. For example, in the Pacific oyster (Crassostrea gigas), SNPs occur, on average, every 40 bp in non-coding regions and every 60 bp in coding regions. Their high frequency in the genome makes SNPs a very efficient type of marker to map or scan genomes. SNPs can be readily identified by sequencing polymerase chain reaction (PCR)-amplified DNA fragments from pooled individuals.
6 Restriction fragment length polymorphism (RFLP) The fact that restriction enzymes will only cut DNA at specific sequences presents us with a simple way of identifying genetic variation caused by point mutations. Let’s say we have a 2-kb length of DNA from an individual animal which can be amplified to a high copy number, and we then incubate this amplified DNA in a microtube with a suite of restriction enzymes. 3). In other individuals of the same species, point mutations will have altered the sequence at one or more restriction enzyme cut sites, or may have produced a cut site where one was not present before.