Download Biotechnology for the Environment: Strategy and Fundamentals by Gerhard Stucki, Patrick Steinle (auth.), Spiros N. Agathos, PDF

By Gerhard Stucki, Patrick Steinle (auth.), Spiros N. Agathos, Walter Reineke (eds.)

At the sunrise of the twenty first century, biotechnology is rising as a key allowing know-how for sustainable environmental safety and stewardship. Biotechnology for the surroundings: procedure and Fundamentals captures the dynamism of environmental biotechnology because it addresses the molecular functioning of microorganisms as cleanup brokers, their communal interactions in normal and polluted ecosystems, and the principles of sensible bioremediation procedures. Chapters on organic pollutants keep watch over within the chemical undefined, biodegradation of chronic molecules (halogenated compounds, polycyclic fragrant hydrocarbons, polychlorinated biphenyls, insecticides, detergents, etc.), microbial range with influence on worldwide swap, bioaugmentation suggestions, and sensors for ecotoxicological tracking, can be of price to environmental scientists, engineers, and decision-makers focused on the advance, assessment, or implementation of organic therapy systems.

For info on Soil Remediation, see Focus on Biotechnology volume3B, and for info on Waste Water and Waste gasoline dealing with, see Focus on Biotechnology quantity 3C.

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Nevertheless, involvement in halorespiratory electron transport has not been investigated. Interestingly, a high spin c-type cytochrome was co-induced with dehalogenation activity in Desulfomonile tiecljei, and the corresponding gene has been cloned and sequenced [45]. The 50-kDa gene product exhibited two conserved c-type heme binding motifs (CxxCH), but substantially differed from known cytochromes and also no homologues could be identified from the Dehalococcoides ethenogenes partial genome sequence.

83. 84. 85. 86. 87. 88. 89. E. M. (1996) Characterization of Desulfitobacterium chlororespirans sp. , which grows by coupling the oxidation of lactate to the reductive dechlorination of3-chloro-4-hydroxybenzoate, Appl. Environ. Microbiol. 62, 3800-3808. , Giordano, G. F. (1998) A novel secindependent periplasmic protein translocation pathway in Escherichia coli, EMBO 1. 17, 101-12. , Moore, E. and Diekert, G. (1995) Isolation and characterization of Dehalospirillum multivorans gen. , sp. , a tetrachloroethene-utilizing, strictly anaerobic bacterium, Arch.

Akkermans, John Van Der Oost and Willem M. De Vos B CI H' lit Figure 3. Proposed reaction mechanisms of (A) corrinoid-containing PCE reductive dehalogenases according to Holliger et al. [30} and (B) o-CP reductive dehalogenase from Desuffitobacterium dehalogenans [86]. Alternatively, Wohlfarth & Diekert [92] proposed that the dehalogenation reaction proceeds through addition of cob(I)alamin to a carbon of the halogenated substrate with subsequent ~-elimination of a chlorine ion. This model assumes the splitting of electrons into one low-potential and one high-potential electron via the two Fe-S clusters.

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